Biotin Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT)

GWB-460460

25-732-294150

2 mg
$210.00
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Description

Anti-Human IgG F(c) Biotin Conjugated generated in goat detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab).

Additional Information

Name Biotin Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT)
Clone Polyclonal
Host Goat
Conjugation Biotin
Immunogen Human IgG F(c) fragment
Product Format Lyophilized from 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2 with 10mg/ml BSA and 0.01% sodium azide.
Datasheets / Downloads GWB-460460 Datasheet
Swiss Prot Number P01857
Applications WB, ELISA, IHC
Application Info ELISA 1:20,000~100,000
WB 1:2,000~10,000
IHC 1:1,000~5,000
Reconstitution and Storage Store vial at 4 C prior to restoration. Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20 C or below.   Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Intended Use Research Use Only
Additional Information Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Color: n/a
Conjugation Reference: Modified from Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
:: Label: Biotin
Preservative: 0.01% (w/v) Sodium Azide
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)  - Immunoglobulin and Protease free