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Biotin Conjugated Affinity Purified Anti-SHEEP IgG (H&L) (RABBIT)



2 mg
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Antigen: IgG

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Fraction: Affinity Purified IgG

Additional Information

Name Biotin Conjugated Affinity Purified Anti-SHEEP IgG (H&L) (RABBIT)
Related Product Names Biotin Conjugated Affinity Purified anti-Sheep IgG [H&L] [Rabbit]; N/A Biotin Conjugated Affinity Purified Anti-SHEEP IgG (H&L) (RABBIT)IGHG1
Swissprot ID P01857
Host Rabbit
Isotype IgG
Conjugation Biotin
Immunogen Sheep IgG whole molecule
Product Format Lyophilized
Molecular Weight 455
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Sheep IgG and Sheep Serum.
Applications WB, ELISA, IHC, IP, Fluorescence Microscopy, FACS
Application Info ELISA: 1:140,000
WB: 1:1,000 - 1:5,000
IHC: 1:500 - 1:3,000
Format Lyophilized
Reconstitution and Storage Store vial at 4 C prior to restoration. Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Reactivity Sheep IgG
Storage 4C
Datasheets/Manuals Printable datasheet for GWB-1C237F
Intended Use Research Use Only
Additional Information Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Conjugation Reference: Modified from Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
:: Preservative: 0.01% (w/v) Sodium Azide
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free