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Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG F(ab')2 (RABBIT)



1.5 mg
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Additional Information

Name Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG F(ab')2 (RABBIT)
Related Product Names Peroxidase Conjugated Affinity Purified anti-Sheep IgG F(ab’)2 [Rabbit]; N/A Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG F(ab\')2 (RABBIT)IGHG1
Alias Symbols HRP
Swissprot ID P01857
Description of Target Anti-Sheep IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the sheep IgG molecule. Sheep IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. F(ab')2 molecules lack the Fc portion of IgG and therefore receptors that bind sheep IgG F(c) will not bind sheep IgG F(ab')2 molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Sheep IgG F(ab')2 is conjugated to Peroxidase.
Clone Polyclonal
Host Rabbit
Immunogen Sheep IgG F(ab')2 fragment
Product Format Lyophilized 0.01M Sodium Phosphate, 0.15M Sodium Chloride (pH 7.2), 10 mg/ml BSA (Immunoglobulin & Protease free) with 0.01% (w/v) Gentamicin Sulfate. Color is substrate dependent
Molecular Weight 40 kDa
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads.
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab’)2 and Sheep Serum. No reaction was observed against Sheep IgG F(c).
Concentration 1.5 mg/ml (UV absorbance at 280 nm, prior to lyophilization)
Applications This product has been assayed against 1.0 µg of Sheep IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Application Info ELISA: 1:10,000 - 1:50,000 WB: 1:1,000 - 1:10,000 IHC: 1:500 - 1:2,500
Reconstitution and Storage Restore with 1.0 mL of deionized water (or equivalent). Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Warning Do NOT add Sodium Azide!
Datasheets/Manuals Printable datasheet for GWB-3E18C4
Intended Use Research Use Only