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CLEC7A ELISA Kit (Human) (GWB-KBBAD3)

GWB-KBBAD3

96 Wells
$640.00
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Additional Information

Name CLEC7A ELISA Kit (Human) (GWB-KBBAD3)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.064 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CLEC7A
Gene id 64581
Alias Symbols Beta-glucan receptor, BGR, CANDF4, CLECSF12, C-type lectin domain family 7 member A, C-type lectin superfamily member 12, DC-associated C-type lectin 1, DECTIN1, dectin-1, Dectin-1, Dendritic cell-associated C-type lectin 1, hDectin-1, UNQ539/PRO1082
Swissprot ID Q9BXN2
Protein Name C-type lectin domain family 7 member A
Description of Target Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation o
Kit Principle Genway Biotech CLEC7A ELISA Kit (Human) (GWB-KBBAD3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLEC7A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLEC7A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLEC7A captured in the well.
Kit Component
ComponentAmount
Anti-CLEC7A Microplate96 Wells (12 x 8 Well Strips)
CLEC7A Lyophilized Standard2 x 10 ng
100X Biotinylated CLEC7A Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_072092
Nucleotide Accession Num NM_022570
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBAD3