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Crp ELISA Kit (Guinea Pig) (GWB-KBBAA2)


96 Wells
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Additional Information

Name Crp ELISA Kit (Guinea Pig) (GWB-KBBAA2)
Kit Range 15.62 - 1,000 pg/mL
Sensitivity < 7.4 pg/mL
Sample Type serum, plasma and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CRP
Gene id 100727100
Alias Symbols C-RP, PTX1, Pentraxin-Related, Pentraxin 1
Swissprot ID P49254
Protein Name C-reactive Protein
Description of Target Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may sc
Kit Principle Genway Biotech Crp ELISA Kit (Guinea Pig) (GWB-KBBAA2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Crp has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Crp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Crp captured in the well.
Kit Component
Anti-CRP Microplate96 Wells (12 x 8 Well Strips)
CRP Lyophilized Standard2 x 1 ng
100X Biotinylated CRP Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num XP_003466601
Nucleotide Accession Num XM_003466553
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)79-94%79-91%94-104%89-104%
EDTA Plasma (n=5)96-103%85-93%81-104%93-101%
Heparin Plasma (n=5)84-99%88-102%79-104%98-105%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)85-9989
EDTA Plasma (n=5)94-10298
Heparin Plasma (n=5)80-9281
Datasheets/Manuals Printable datasheet for GWB-KBBAA2