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Ctss ELISA Kit (Mouse) (GWB-KBBAC2)

GWB-KBBAC2

96 Wells
$535.00
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Additional Information

Name Ctss ELISA Kit (Mouse) (GWB-KBBAC2)
Gene Symbol CTSS
Gene id 13040
Alias Symbols Cathepsin S, Cats, Cat S
Protein Name cathepsin S
Description of Target Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.
Swissprot ID O70370
Protein Accession Num NP_067256
Nucleotide Accession Num NM_021281
Species Reactivity Mouse
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Sensitivity < 12.2 pg/mL
Kit Range 31.25 - 2,000 pg/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Datasheets / Downloads GWB-KBBAC2 Datasheet
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Principle Genway Biotech Ctss ELISA Kit (Mouse) (GWB-KBBAC2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ctss has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ctss is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ctss captured in the well.
Kit Component
ComponentAmount
Anti-Cer1 Microplate96 Wells (12 x 8 Well Strips)
Cer1 Lyophilized Standard2 x 2 ng
100X Biotinylated Cer1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Colorimetric, OD 450 nm
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)96-104%90-103%84-97%94-101%
EDTA Plasma (n=5)78-99%81-98%82-94%80-101%
Heparin Plasma (n=5)90-101%81-90%87-105%84-97%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)90-9793
EDTA Plasma (n=5)91-10196
Heparin Plasma (n=5)84-9188