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In observance of Memorial Day, Genway Biotech office will be closed on Monday, May 28th, 2018

Dkk4 ELISA Kit (Mouse) (GWB-KBBAA7)

GWB-KBBAA7

96 Wells
$600.00
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Additional Information

Name Dkk4 ELISA Kit (Mouse) (GWB-KBBAA7)
Gene Symbol DKK4
Gene id 234130
Alias Symbols Dickkopf-4, Dickkopf-related protein 4, Dkk-4, MGC25705
Protein Name dickkopf-related protein 4
Description of Target Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they
Swissprot ID Q8VEJ3
Protein Accession Num NP_663567
Nucleotide Accession Num NM_145592
Species Reactivity Mouse
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Sensitivity < 0.051 ng/mL
Kit Range 0.156 - 10 ng/mL
Sample Type serum, plasma and other biological fluids.
Datasheets / Downloads GWB-KBBAA7 Datasheet
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Principle Genway Biotech Dkk4 ELISA Kit (Mouse) (GWB-KBBAA7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dkk4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Dkk4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dkk4 captured in the well.
Kit Component
ComponentAmount
Anti-CHL1 Microplate96 Wells (12 x 8 Well Strips)
CHL1 Lyophilized Standard2 x 10 ng
100X Biotinylated CHL1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Colorimetric, OD 450 nm
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)89-98%94-105%93-101%91-98%
EDTA Plasma (n=5)79-104%86-102%97-104%78-88%
Heparin Plasma (n=5)78-98%95-102%88-98%94-101%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)95-10299
EDTA Plasma (n=5)97-104101
Heparin Plasma (n=5)92-10196