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Il2 ELISA Kit (Rabbit) (GWB-KBBAB9)


96 Wells
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Additional Information

Name Il2 ELISA Kit (Rabbit) (GWB-KBBAB9)
Kit Range 15.6 - 1,000 pg/mL
Sensitivity < 6.2 pg/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol IL2
Gene id 100302458
Alias Symbols IL2, Interleukin-2
Swissprot ID O77620
Protein Name Interleukin-2
Description of Target Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells
Kit Principle Genway Biotech Il2 ELISA Kit (Rabbit) (GWB-KBBAB9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Il2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Il2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Il2 captured in the well.
Kit Component
Anti-IL2 Microplate96 Wells (12 x 8 Well Strips)
IL2 Lyophilized Standard2 x 1 ng
100X Biotinylated IL2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001156652
Nucleotide Accession Num NM_001163180
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)80-104%88-97%88-98%78-93%
EDTA Plasma (n=5)83-104%94-105%91-99%79-91%
Heparin Plasma (n=5)83-92%80-95%88-105%82-99%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)79-9388
EDTA Plasma (n=5)95-103101
Heparin Plasma (n=5)79-9792
Datasheets/Manuals Printable datasheet for GWB-KBBAB9