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Lrp5 ELISA Kit (Rat) (GWB-KBBAA4)


96 Wells
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Additional Information

Name Lrp5 ELISA Kit (Rat) (GWB-KBBAA4)
Kit Range 0.781 - 50 ng/mL
Sensitivity < 0.30 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol LRP5
Gene id 293649
Alias Symbols HBM, BMND1, EVR1, EVR4, LR3, LRP7, OPPG, OPS, OPTA1, VBCH2, Osteoporosis Pseudoglioma Syndrome, Exudative Vitreoretinopathy 1
Swissprot ID B2RYI1
Protein Name low-density lipoprotein receptor-related protein 5
Kit Principle Genway Biotech Lrp5 ELISA Kit (Rat) (GWB-KBBAA4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lrp5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lrp5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lrp5 captured in the well.
Kit Component
Anti-HACD1 Microplate96 Wells (12 x 8 Well Strips)
HACD1 Lyophilized Standard2 x 50 ng
100X Biotinylated HACD1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001099791
Nucleotide Accession Num NM_001106321
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBAA4