MUTYH ELISA Kit (Human) (GWB-KBBAC6)

GWB-KBBAC6

96 Wells
$580.00
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Additional Information

Name MUTYH ELISA Kit (Human) (GWB-KBBAC6)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.057 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol MUTYH
Gene id 4595
Alias Symbols A/G-specific adenine DNA glycosylase, CYP2C, hMYH, MGC4416, MutY homolog, MYH
Swissprot ID Q9UIF7
Protein Name adenine DNA glycosylase
Description of Target Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities.
Kit Principle Genway Biotech MUTYH ELISA Kit (Human) (GWB-KBBAC6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MUTYH has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MUTYH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MUTYH captured in the well.
Kit Component
ComponentAmount
Anti-Rab1A Microplate96 Wells (12 x 8 Well Strips)
Rab1A Lyophilized Standard2 x 10 ng
100X Biotinylated Rab1A Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001041636
Nucleotide Accession Num NM_001048171
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Datasheets / Downloads GWB-KBBAC6 Datasheet
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours