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96 Wells
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Additional Information

Name NCF1 ELISA Kit (Human) (GWB-KBBAE1)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.113 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol NCF1
Gene id 653361
Alias Symbols NCF1A, NOXO2, SH3PXD1A, p47-phox, NCF-47K, 47 kDa autosomal chronic granulomatous disease protein, Neutrophil NADPH oxidase factor 1, Nox-organizing protein 2
Swissprot ID P14598
Protein Name neutrophil cytosol factor 1
Description of Target NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production)
Kit Principle Genway Biotech NCF1 ELISA Kit (Human) (GWB-KBBAE1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NCF1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NCF1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NCF1 captured in the well.
Kit Component
Anti-OSCAR Microplate96 Wells (12 x 8 Well Strips)
OSCAR Lyophilized Standard2 x 20 ng
100X Biotinylated OSCAR Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000256
Nucleotide Accession Num NM_000265
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBAE1