Rbp4 ELISA Kit (Mouse) (GWB-KBBAC9)

GWB-KBBAC9

96 Wells
$380.00
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Additional Information

Name Rbp4 ELISA Kit (Mouse) (GWB-KBBAC9)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.142 ng/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol RBP4
Gene id 19662
Alias Symbols PRBP, RBP, Plasma retinol-binding protein
Swissprot ID H7BWY6
Protein Name retinol-binding protein 4
Kit Principle Genway Biotech Rbp4 ELISA Kit (Mouse) (GWB-KBBAC9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rbp4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Rbp4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rbp4 captured in the well.
Kit Component
ComponentAmount
Anti-MPZ Microplate96 Wells (12 x 8 Well Strips)
MPZ Lyophilized Standard2 x 20 ng
100X Biotinylated MPZ Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001152959
Nucleotide Accession Num NM_001159487
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBAC9