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In observance of Memorial Day, Genway Biotech office will be closed on Monday, May 28th, 2018

Ryr1 ELISA Kit (Rat) (GWB-KBBAB4)

GWB-KBBAB4

96 Wells
$580.00
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Additional Information

Name Ryr1 ELISA Kit (Rat) (GWB-KBBAB4)
Gene Symbol RYR1
Gene id 114207
Alias Symbols Ryr1, Ryanodine receptor 1
Protein Name Ryanodine receptor 1
Description of Target Calcium channel that mediates the release of Ca2+ from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules. Repeated very high-level exercise increases the open
Swissprot ID F1LMY4
Protein Accession Num XP_017445931
Nucleotide Accession Num XM_017604710
Species Reactivity Rat
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Sensitivity < 0.248 ng/mL
Kit Range 0.625 - 40 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Datasheets / Downloads GWB-KBBAB4 Datasheet
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Principle Genway Biotech Ryr1 ELISA Kit (Rat) (GWB-KBBAB4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ryr1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ryr1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ryr1 captured in the well.
Kit Component
ComponentAmount
Anti-Ryr1 Microplate96 Wells (12 x 8 Well Strips)
Ryr1 Lyophilized Standard2 x 40 ng
100X Biotinylated Ryr1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Colorimetric, OD 450 nm
Kit Duration ~ 3 Hours