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Ryr1 ELISA Kit (Rat) (GWB-KBBAB4)


96 Wells
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Additional Information

Name Ryr1 ELISA Kit (Rat) (GWB-KBBAB4)
Kit Range 0.625 - 40 ng/mL
Sensitivity < 0.248 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol RYR1
Gene id 114207
Alias Symbols Ryr1, Ryanodine receptor 1
Swissprot ID F1LMY4
Protein Name Ryanodine receptor 1
Description of Target Calcium channel that mediates the release of Ca2+ from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules. Repeated very high-level exercise increases the open
Kit Principle Genway Biotech Ryr1 ELISA Kit (Rat) (GWB-KBBAB4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ryr1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ryr1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ryr1 captured in the well.
Kit Component
Anti-Ryr1 Microplate96 Wells (12 x 8 Well Strips)
Ryr1 Lyophilized Standard2 x 40 ng
100X Biotinylated Ryr1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num XP_017445931
Nucleotide Accession Num XM_017604710
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBAB4