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Biotin Conjugated Affinity Purified Anti-RABBIT IgG F(c) (GOAT) (Min X Human Serum Proteins)



2 mg
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Additional Information

Name Biotin Conjugated Affinity Purified Anti-RABBIT IgG F(c) (GOAT) (Min X Human Serum Proteins)
Alias Symbols Goat anti-Rabbit IgG F(c) Antibody biotin Conjugation, Goat anti-Rabbit IgG Fc fragment Antibody biotin Conjugation, Goat anti-Rabbit IgG F(c) BAC Conjugated Antibody
Swissprot ID P01857
Description of Target Anti-Rabbit IgG F(c) Biotin antibody generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Anti-RABBIT IgG F(c) (GOAT) Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
Clone Polyclonal
Host Goat
Isotype IgG
Conjugation Biotin
Immunogen Rabbit IgG F(c) fragment
Product Format Lyophilized from 0.02M Potassium Phosphate, 0.15M Sodium Chloride (pH 7.2), 10 mg/mL BSA (immunoglobulin & protease free) with 0.01% (w/v) Sodium Azide
Molecular Weight 455
Purification Anti-RABBIT IgG F(c) (GOAT) was purified from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab) or Human Serum Proteins.
Concentration 2 mg/ml (UV absorbance at 280nm) (prior to lyophilization)
Applications ELISA: 1:200000
IHC: 1:1000~5000
WB: 1:2000~10000
Anti-RABBIT IgG F(c) (GOAT) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.
All assays should be optimized by the end user.
Application Info ELISA: 1:115,000 WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000
Stability Expiration: One (1) year from date of opening.
Reconstitution and Storage Restore with 1.0 mL of deionized water (or equivalent). Reconstitution volume: 1.0 mL
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Datasheets/Manuals Printable datasheet for GWB-18FD40