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96 Wells
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Additional Information

Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.055 ng/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol SERPINB4
Gene id 6318
Alias Symbols SERPINB4, PI11, SPB4, Leupin, Serpin Peptidase Inhibitor,Clade B,Member 4, Peptidase Inhibitor 11
Swissprot ID Q86W05
Protein Name serpin B4 isoform 1
Kit Principle Genway Biotech SERPINB4 ELISA Kit (Human) (GWB-KBBBA8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SERPINB4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SERPINB4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SERPINB4 captured in the well.
Kit Component
Anti-Pgc Microplate96 Wells (12 x 8 Well Strips)
Pgc Lyophilized Standard2 x 10 ng
100X Biotinylated Pgc Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_002965
Nucleotide Accession Num NM_002974
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)78-101%86-99%99-105%92-99%
EDTA Plasma (n=5)93-101%87-98%79-96%95-104%
Heparin Plasma (n=5)96-105%85-93%78-98%80-102%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)78-9586
EDTA Plasma (n=5)97-105102
Heparin Plasma (n=5)83-9086
Datasheets/Manuals Printable datasheet for GWB-KBBBA8
Species Reactivity Human