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Serpinc1 ELISA Kit (Rat) (GWB-KBBHS9)

This exact product is now provided by Aviva Systems Biology.
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96 Wells
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Additional Information

Name Serpinc1 ELISA Kit (Rat) (GWB-KBBHS9)
Kit Range 3.12 - 200 ng/mL
Sensitivity < 1.38 ng/mL
Sample Type serum, plasma and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol SERPINC1
Gene id 304917
Alias Symbols AT3, ATIII, SERPINC1, Anti-Thrombin Antibodies, Serpin Peptidase Inhibitor Clade C Member 1, Coding Sequence Signal Peptide Antithrombin Part 1
Swissprot ID Q5M7T5
Protein Name antithrombin-III
Kit Principle Genway Biotech Serpinc1 ELISA Kit (Rat) (GWB-KBBHS9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpinc1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Serpinc1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serpinc1 captured in the well.
Kit Component
Anti-Pcsk1 Microplate96 Wells (12 x 8 Well Strips)
Pcsk1 Lyophilized Standard2 x 200 ng
100X Biotinylated Pcsk1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_543120
Nucleotide Accession Num NM_080844
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)87-94%83-90%92-105%92-105%
EDTA Plasma (n=5)89-96%81-103%83-99%93-101%
Heparin Plasma (n=5)94-102%99-105%90-102%92-105%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)80-9380
EDTA Plasma (n=5)79-9995
Heparin Plasma (n=5)98-105102
Datasheets/Manuals Printable datasheet for GWB-KBBHS9
Species Reactivity Rat