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Serpinf1 ELISA Kit (Rat) (GWB-KBBLO7)

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96 Wells
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Additional Information

Name Serpinf1 ELISA Kit (Rat) (GWB-KBBLO7)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.059 ng/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol SERPINF1
Gene id 287526
Alias Symbols Dmrs91, Pedf
Swissprot ID Q80ZA3
Protein Name pigment epithelium-derived factor
Kit Principle Genway Biotech Serpinf1 ELISA Kit (Rat) (GWB-KBBLO7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpinf1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Serpinf1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serpinf1 captured in the well.
Kit Component
Anti-SLC7A5 Microplate96 Wells (12 x 8 Well Strips)
SLC7A5 Lyophilized Standard2 x 10 ng
100X Biotinylated SLC7A5 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_808788
Nucleotide Accession Num NM_177927
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)84-99%86-93%96-105%93-104%
EDTA Plasma (n=5)91-103%89-101%82-93%95-105%
Heparin Plasma (n=5)88-101%87-96%87-94%78-102%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)78-9383
EDTA Plasma (n=5)87-10197
Heparin Plasma (n=5)88-9992
Datasheets/Manuals Printable datasheet for GWB-KBBLO7
Species Reactivity Rat