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96 Wells
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Additional Information

Kit Range 0.781 - 50 ng/mL
Sensitivity < 0.34 ng/mL
Sample Type serum, plasma and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol SERPINI1
Gene id 5274
Alias Symbols DKFZp781N13156, neuroserpin, Neuroserpin, Peptidase inhibitor 12, PI12, PI-12, Serpin I1
Swissprot ID Q99574
Protein Name neuroserpin
Description of Target Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons fro
Kit Principle Genway Biotech SERPINI1 ELISA Kit (Human) (GWB-KBBOA5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SERPINI1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SERPINI1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SERPINI1 captured in the well.
Kit Component
Anti-Irs2 Microplate96 Wells (12 x 8 Well Strips)
Irs2 Lyophilized Standard2 x 50 ng
100X Biotinylated Irs2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001116224
Nucleotide Accession Num NM_001122752
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)83-103%94-105%81-96%78-98%
EDTA Plasma (n=5)79-102%88-102%89-102%85-93%
Heparin Plasma (n=5)95-102%97-105%97-104%86-97%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)91-10596
EDTA Plasma (n=5)88-9591
Heparin Plasma (n=5)92-10599
Datasheets/Manuals Printable datasheet for GWB-KBBOA5
Species Reactivity Human