| Name |
SERPINI1 ELISA Kit (Human) (GWB-KBBOA5) |
| Kit Range |
0.781 - 50 ng/mL |
| Sensitivity |
< 0.34 ng/mL |
| Sample Type |
serum, plasma and other biological fluids |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
SERPINI1 |
| Gene id |
5274 |
| Alias Symbols |
DKFZp781N13156, neuroserpin, Neuroserpin, Peptidase inhibitor 12, PI12, PI-12, Serpin I1 |
| Swissprot ID |
Q99574 |
| Protein Name |
neuroserpin |
| Description of Target |
Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons fro |
| Kit Principle |
Genway Biotech SERPINI1 ELISA Kit (Human) (GWB-KBBOA5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SERPINI1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SERPINI1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SERPINI1 captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-Irs2 Microplate | 96 Wells (12 x 8 Well Strips) | | Irs2 Lyophilized Standard | 2 x 50 ng | | 100X Biotinylated Irs2 Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_001116224 |
| Nucleotide Accession Num |
NM_001122752 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Kit linearity |
| Sample | 1:2 Dilution | 1:4 Dilution | 1:8 Dilution | 1:16 Dilution |
|---|
| Serum (n=5) | 83-103% | 94-105% | 81-96% | 78-98% | |
| EDTA Plasma (n=5) | 79-102% | 88-102% | 89-102% | 85-93% | |
| Heparin Plasma (n=5) | 95-102% | 97-105% | 97-104% | 86-97% | |
|
| Kit Recovery |
| Matrix | Recovery Range (%) | Average (%) |
|---|
| Serum (n=5) | 91-105 | 96 | |
| EDTA Plasma (n=5) | 88-95 | 91 | |
| Heparin Plasma (n=5) | 92-105 | 99 | |
|
| Datasheets/Manuals |
Printable datasheet for GWB-KBBOA5 |
| Species Reactivity |
Human |
