Custom Antibody Services
GenWay offers first-in-class custom antibody services. Our team excels in the two key factors for producing high quality antibodies: Antigen design and Antibody purification. Services include Polyclonal IgY, Polyclonal IgG, Phospho-Specific antibodies, Monoclonal IgY, Hybridoma Development and Monoclonal IgG production. Please use our convenient online Antibody Development Quote Form if you would get a quote or schedule a call to discuss your needs.
GenWay Biotech has over 15 years of experience in development and production of monoclonal antibodies. Our experienced scientific team will work to design a protocol that will meet your needs for research, product development, or Clinical or IVD use.
Standard Hybridoma Development Description
I. Immunization
- Purchase of 5 Balb/C mice or 3 Fisher rats
- Pre-immunization test bleed for negative baseline
- Immunizations and test bleeds
- Antibody titer of test bleeds by ELISA
- One or two mice or rats with the best titers are selected for fusion
- Pre-spleen collection boost before fusing
- Typically 70 – 90 days depending on antigen and immune response
- Lymphocytes from 2 mice or one rat are pooled and fused.
- Fused cells are grown on HAT selective single-step cloning media.
- Clones are transferred to 96-well plates and expanded.
- 10 - 14 days
- Primary screening of hybridoma tissue culture supernatants by indirect ELISA.
- Positive clones are further tested by ELISA.
- Positive clones are isotyped and subcloned to identify stable expressing clones.
- Further evaluation by the client if needed (ship supertantant to client).
- Report to client
- 14 - 21 Days
- Selection of 1 to 5 clones in conjunction with client.
- Cryopreservation of at least 5 vials per selected clone.
- Storage of vials for 3 months at no charge.
- Long term storage available (optional).
- Report to client
- 14 - 21 days
- GenWay offers antibody production and purification services to meet the needs of research, diagnostic development, or for use in FDA or CE medical devices