In observance of Thanksgiving, Genway Biotech office will be closed on Thursday and Friday, November 22-23, 2018

Custom Antibody Services

GenWay offers first-in-class custom antibody services. Our team excels in the two key factors for producing high quality antibodies: Antigen design and Antibody purification. Services include Polyclonal IgY, Polyclonal IgG, Phospho-Specific antibodies, Monoclonal IgY, Hybridoma Development and Monoclonal IgG production. Please use our convenient online Antibody Development Quote Form if you would get a quote or schedule a call to discuss your needs.

Contact us and receive a quote within 24 hours
  • Email: sales@genwaybio.com
  • Phone: 858.458.0866
  • Phospho-Specific Antibody Development The key to generating phospho-specific antibodies is to design specific and immunogenic antigens. GenWay has successfully produced phospho-specific antibodies that are specific to the phosphorylation sites of interest to research scientists. GenWay’s phospho-specific antibody development service includes the following steps: Antibody Design and Peptide Synthesis The common antigen for a phospho-specific antibody is a synthetic peptide carrying one or two phospho residues, tyrosine, threonine, or serine. Clients provide information about the desired phospho residue and its flanking sequence, then GenWay will design the phospho-peptide. Two peptides will be synthesized, a phospho-peptide and a non-phospho-peptide. Antibody Generation Antibody production will use GenWay's expertise in producing polyclonal antibodies. Antibodies from egg yolk (or IgG in serum) will be prepared and further purified. Phospho-Specific Purification Immunization with a phosphopeptide may generate two different types of antibodies:
    1. Phospho-specific antibodies
    2. Pan-specific antibodies against the non-phosphoryl polypeptide
    Two antigen-affinity columns will be used to purify the phospho-specific antibodies and pan-specific antibodies. The first column to be used is the non-phosphopeptide affinity column. Antibodies recognizing the non-phospho residues of the peptide will bind to the column and be eluted as pan-specific antibodies. The flow-through fraction contains non-specific antibodies and antibodies that are specific to the phospho-residue. The collected flow-through fraction will then be applied to the second column, the phospho-peptide column. Antibodies recognizing the phospho-residue will bind to the column and then be eluted as phospho-specific antibodies. Non-specific antibodies will be removed as flow-through. Antibody Characterization Initial ELISA characterization will test the degree of cross reactivity of the two products toward each substrate. In general, there should be less than 0.1% binding of non-phospho peptide compared to the targeted phosphorylated peptide. Subsequent testing can involve WB, IHC, ICC, etc, for analyses of the antibodies produced, using available cell lines or samples provided by client. GenWay will collaborate with the client to design immunogen sequences and develop the specific project protocol. Each project delivers phospho-specific and pan-specific antibodies, antigen samples, pre-immune serum and total antibody. GenWay will collaborate with the client to design immunogen sequences and develop the specific project protocol. Each project delivers phospho-specific and pan-specific antibodies, antigen samples, pre-immune serum and total antibody.