Protein Expression Services

GenWay provides customized solutions to each protein expression project. Our expert scientific staff has experience with difficult proteins and have produced more than 2,500 recombinant proteins. Whether your needs are small scale research or large scale cGMP, we can apply our experience to meet your needs. Services include E. coli expression, Mammalian expression, Yeast expression, and Antigen Preparation. Please use our convenient online Protein Expression Quote Form if you would get a quote or schedule a call to discuss your needs.

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  • Phone: 858.458.0866
  • Mammalian Protein Expression Services GenWay mammalian expression systems generate high-yields of mammalian proteins. These expression systems are often suggested for proteins requiring mammalian post-translational modifications. GenWay has a great amount of experience producing monoclonal antibodies or recombinant proteins from a wide range of cell types including CHO, hybridomas, various mouse myeloma, and human cell lines. GenWay performs a comprehensive analysis of your project in order to identify the best expression platforms with appropriate scalability, yield, and functionality. Our service includes bioinformatics analysis, domain selection, vector design, tag selection, sequence confirmation, cell transfection, cell amplification, purification by tag selection, yield optimization, scale up processing, and protein characterization. View our ISO13485 compliant protein development, validation, and production options. The following is our mammalian protein expression development template:
    Order Process Details
    Step 1 Bioinformatic analysis, domain selection and vector design GenWay conducts bioinformatic analysis on target(s) and recommends an expression strategy to each client. Sequence verified cDNAs are provided by the client, supplied by GenWay, or are purchased from available commercial sources.
    Step 2 Construction of expression vectors The target sequence will be cloned into GenWay’s vector system. Based on the specific target protein, the sequence can be inserted into an expression cassette for a secreted or non-secreted protein. GenWay will conduct DNA sequencing and analysis to confirm correct coding and flanking sequences.
    Step 3 Cell transfection, selection, and cloning of target-expressing cells The protein can be produced by transient burst or stable cell line expression depending on the client’s specific needs. The expression of the target protein will be confirmed by SDS page or Western blot using an anti-tag antibody or client-provided antibody against the target protein.
    Step 4 Protein production and purification GenWay will express the protein in a scale of up to 4x T-75 culture flasks or equivalent. The protein will be purified using a tag-assisted affinity column or other chromatographic methods. Protein samples may be provided to clients if the protein function needs to be confirmed.
    Step 5 Scale-up production and quality control test GenWay will produce the target protein(s) at large scale. The scale of production depends on the level of protein expression, the yield of purification, and the requirements of the client.
    Step 6 Characterization of target protein (including functional studies) 1. Determine purity in conjunction with client specifications.
    2. Employ a functional assay method provided by client or to be specifically developed.
    3. Utilize immunoassays such as western blot analysis, ELISA, etc, after antibodies are produced or provided.
    Each protein has its unique biochemical feature in the expression process. GenWay's team will work with the client to develop the best strategy and use the most cost-effective process. Purification Methods
    1. Affinity Chromatography
      • Anti-Flag
      • Glutathione Sepharose
      • Heparin Sepharose
      • Ni-NTA
      • Protein A
      • Protein G
      • Streptavidin Sepharose
      • Talon resin
    2. Ion Exchange Chromatography
      • ANX
      • CM Sepharose
      • DEAE Sepharose
      • Mono-Q
      • Q Sepharose Fast Flow
      • Q Sepharose XL
      • Resource Q
      • SP Sepharose
    3. Hydrophobic Interaction Chromatography
      • (CH2)n Sepharose
      • Phenyl Sepharose
    4. Size Exclusion Chromatography
    Optional Services
    1. Tag Removal
    2. Endotoxin Removal
    3. Protein solubilization and refolding