Protein Expression Services

GenWay provides customized solutions to each protein expression project. Our expert scientific staff has experience with difficult proteins and have produced more than 2,500 recombinant proteins. Whether your needs are small scale research or large scale cGMP, we can apply our experience to meet your needs. Services include E. coli expression, Mammalian expression, Yeast expression, and Antigen Preparation. Please use our convenient online Protein Expression Quote Form if you would get a quote or schedule a call to discuss your needs.

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  • Yeast Protein Expression Services GenWay’s yeast expression platform provides a flexible, controllable, and effective solution for protein expression needs. GenWay’s various yeast expression platforms have been well characterized and are validated to perform many post-translational modifications. GenWay offers an initial assessment of your project and makes recommendations regarding expression platforms with appropriate scalability, yield, and functionality. Our approach includes bioinformatics analysis, domain selection, vector design, tag selection, sequence confirmation, cell transfection, cell amplification, purification by tag selection, yield optimization, scale up processing, and protein characterization View our ISO13485 compliant protein development, validation, and production options. The following is our Yeast protein expression development template:
    Order Process Details
    Step 1 Bioinformatic analysis, domain selection and vector design GenWay conducts bioinformatic analysis on target(s) and recommends an expression strategy to each client. Sequence verified cDNAs are provided by the client, supplied by GenWay, or are purchased from available commercial sources.
    Step 2 Prepare subcloning plasmid and construct expression vectors Project design includes GenWay’s vector system with the most appropriate strategy to construct vectors. If certain specific vectors are requirgb(170,0,0), then custom vectors will be included in the project design.
    Step 3 Target sequence analysis and confirmation Conduct DNA sequencing and analysis to confirm correct coding and flanking sequence.
    Step 4 Cell transformation and expression clone preparation GenWay will utilize proprietary expression cell lines and prepare expression cell clone. If specific cell lines (e.g. mutants) are needed for accommodating the need for recombinant protein expression and selection, GenWay’s team will discuss with client to make specific arrangements for the project.
    Step 5 Protein production and purification Express protein in a small scale culture. Fermentation broth or cell lysate (prepargb(170,0,0) by using microfluidizer) will be collected for filtration and concentration. Expressed protein will be further isolated and purified according the protocol provided by client or developed by GenWay. If the protein is not soluble, processes of solubilization and refolding will be initiated.
    Step 6 Quality control test Conduct SDS-PAGE analysis and generate QC report.
    Step 7 Scale-up production and quality control test Produce and QC target proteins at a large scale according to client requirements.
    Step 8 Characterization of target protein (including functional studies) 1. Determine purity in conjunction with client specifications.
    2. Employ a functional assay method provided by client or to be specifically developed.
    3. Utilize immunoassays such as western blot analysis, ELISA, etc, after antibodies are produced or provided.
    Each protein has its unique biochemical feature in the expression process. GenWay's team will work with the client to develop the best strategy and use the most cost-effective process. Purification Methods
    1. Affinity Chromatography
      • Anti-Flag
      • Glutathione Sepharose
      • Heparin Sepharose
      • Ni-NTA
      • Protein A
      • Protein G
      • Streptavidin Sepharose
      • Talon resin
    2. Ion Exchange Chromatography
      • ANX
      • CM Sepharose
      • DEAE Sepharose
      • Mono-Q
      • Q Sepharose Fast Flow
      • Q Sepharose XL
      • Resource Q
      • SP Sepharose
    3. Hydrophobic Interaction Chromatography
      • (CH2)n Sepharose
      • Phenyl Sepharose
    4. Size Exclusion Chromatography
    Optional Services
    1. Tag Removal
    2. Endotoxin Removal
    3. Protein solubilization and refolding