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Sptan1 ELISA Kit (Mouse) (GWB-KBBGZ7)

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Additional Information

Name Sptan1 ELISA Kit (Mouse) (GWB-KBBGZ7)
Description of Target Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Kit Range 1.56 - 100 ng/mL
Sensitivity < 0.68 ng/mL
Sample Type tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol SPTAN1
Gene id 20740
Alias Symbols 2610027H02Rik, alpha-fodrin, Alpha-II spectrin, brain, Fodrin alpha chain, Spectrin, non-erythroid alpha chain, Spectrin alpha chain, brain, Spna-2, Spta2, Sptan1
Swissprot ID P16546
Protein Name Spectrin alpha chain, non-erythrocytic 1
Kit Principle Genway Biotech Sptan1 ELISA Kit (Mouse) (GWB-KBBGZ7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sptan1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Sptan1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Sptan1 captured in the well.
Kit Component
Anti-AKT3 Microplate96 Wells (12 x 8 Well Strips)
AKT3 Lyophilized Standard2 x 100 ng
100X Biotinylated AKT3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001171138
Nucleotide Accession Num NM_001177667
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBGZ7
Species Reactivity Mouse