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96 Wells
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Additional Information

Name SPTbN4 ELISA Kit (Human) (GWB-KBBJC4)
Description of Target Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is compo
Kit Range 0.156-10 ng/mL
Sensitivity < 0.063 ng/mL
Sample Type Serum, plasma, tissue homogenates, cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol SPTbN4
Gene id 57731
Alias Symbols Beta-IV spectrin, KIAA1642, QV, Spectrin, non-erythroid beta chain 3, Spectrin beta chain, brain 3, SPNB4, SPTBN3
Swissprot ID Q9H254
Protein Name Spectrin beta chain, non-erythrocytic 4
Kit Principle Genway Biotech SPTbN4 ELISA Kit (Human) (GWB-KBBJC4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SPTbN4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SPTbN4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SPTbN4 captured in the well.
Kit Component
Anti-SPTbN4 Microplate96 Wells (12 x 8 Well Strips)
SPTbN4 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X SPTbN4 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_066022.2
Nucleotide Accession Num NM_020971.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBJC4
Species Reactivity Human