TBXA2R ELISA Kit (Human) (GWB-KBBQG6) (Aviva Cat. No. OKCD00353)

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GWB-KBBQG6

Aviva Cat. No.: OKCD00353

Additional Information

Name TBXA2R ELISA Kit (Human) (GWB-KBBQG6)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.055 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol TBXA2R
Gene id 6915
Alias Symbols Prostanoid TP receptor, Thromboxane A2 receptor, TXA2-R
Swissprot ID P21731
Protein Name thromboxane A2 receptor
Description of Target Receptor for thromboxane A2 (TXA2), a potent stimulator of platelet aggregation. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. In the kidney, the binding of TXA2 to glomerul
Kit Principle Genway Biotech TBXA2R ELISA Kit (Human) (GWB-KBBQG6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TBXA2R has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TBXA2R is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TBXA2R captured in the well.
Kit Component
ComponentAmount
Anti-TPP1 Microplate96 Wells (12 x 8 Well Strips)
TPP1 Lyophilized Standard2 x 10 ng
100X Biotinylated TPP1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001051
Nucleotide Accession Num NM_001060
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBQG6
Species Reactivity Human