Protein Expression Services
GenWay provides customized solutions to each protein expression project. Our expert scientific staff has experience with difficult proteins and have produced more than 2,500 recombinant proteins. Whether your needs are small scale research or large scale cGMP, we can apply our experience to meet your needs. Services include E. coli expression, Mammalian expression, Yeast expression, and Antigen Preparation. Please use our convenient online Protein Expression Quote Form if you would get a quote or schedule a call to discuss your needs.
Antigen Preparation Services
One of the determining factors in quality antibody development is quality antigen preparation. We focus on preparation of high-quality antigen for ensuring high-quality antibody development
GenWay’s antigen technology specializes in the three major types:
Purification Methods
- Using our bioinformatics core we determine the best immunogenic domain for antibody generation
- Using our proprietary fusion domain and vectors we express and purify recombinant antigens in the most cost-effective manner
- GenWay can provide full services, with the customer providing a target protein accession number and GenWay delivering high-quality polyclonal or monoclonal antibodies
| Comparison of Antigens | ||||
| Consideration | Native Proteins | Recombinant Proteins | Peptides | Other |
| Size and form | Full-Length Vary in size | 50-300 aa (Domain) or Full-Length | 6-20 aa | Small molecule, macromolecule whole cell or microorganism |
| Conjugation (Carrier) | Not Needed | Not Needed | Needed | Needed for small and macromolecule |
| Fusion Tag | No | Optional | No | Optional |
| Immunogenicity | Best | High | Fair | Fair |
| Success Rate for Antibody Generation | 80-95% | 60-80% | 30-60% | 40-60% |
| Suitable as Ligand | Best | Good | Fair | Vary |
| Immuno-reactivity | Good | Good | Vary | Vary |
| Reproducibility | Best | Good | Good | Vary |
| Cost | High | Middle | Low | Vary |
- Native Proteins
- Peptides and Conjugates
- Recombinant Proteins