Custom Antibody Services

GenWay offers first-in-class custom antibody services. Our team excels in the two key factors for producing high quality antibodies: Antigen design and Antibody purification. GenWay has the technology, experience, and methods to deliver the custom antibody fit to your needs.

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  • IgY Monoclonal Antibody Development GenWay Biotech is the only company currently offering custom scFv chicken IgY development. Chicken IgY scFv fragments combine the advantages of chicken IgY polyclonal antibodies, monoclonal antibodies, and scFv recombinant antibodies. Recombinant scFv single-chain antibodies are gaining popularity in the therapeutic and diagnostic industries. Advantages of scFv antibodies include:
    1. Higher specificity
    2. Alterable affinity after clone selection by random mutagenesis
    3. Highly scalable manufacturing - fragments are expressed in bacteria and purified using regular protein tags
    4. Lower cross-reactivity due to the absence of invariable regions
    5. Consistency and reproducibility
    Our general process for scFV antibody development is as follows: Antigen is prepared using bioinformatics analysis and our proprietary fusion domains and vectors. Chickens are immunized and polyclonal antibodies are analyzed to indicate immune response of the host. Once the chicken with the best immune response is identified, its B-cells are isolated, and total RNA is prepared. Total RNA is later used for cDNA library generation and subsequent phage library preparation. Positive clones are selected and scFv fragments are expressed in E. coli. Process Summary
    1. Immunization and Purification of Polyclonal IgY
    2. IgY Fragment coding cDNA preparation
    3. Phage library construction
    4. Phage library screening
    5. Antibody expression in E. coli
    6. Antibody characterization