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TLR5 ELISA Kit (Human) (GWB-KBBAZ2)

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GWB-KBBAZ2

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Additional Information

Name TLR5 ELISA Kit (Human) (GWB-KBBAZ2)
Description of Target Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Kit Range 0.781 - 50 ng/mL
Sensitivity < 0.32 ng/mL
Sample Type tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol TLR5
Gene id 7100
Alias Symbols FLJ10052, MGC126430, MGC126431, SLEB1, TIL3, Toll/interleukin-1 receptor-like protein 3, Toll-like receptor 5
Swissprot ID O60602
Protein Name toll-like receptor 5
Kit Principle Genway Biotech TLR5 ELISA Kit (Human) (GWB-KBBAZ2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TLR5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TLR5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TLR5 captured in the well.
Kit Component
ComponentAmount
Anti-Gad1 Microplate96 Wells (12 x 8 Well Strips)
Gad1 Lyophilized Standard2 x 50 ng
100X Biotinylated Gad1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_003259
Nucleotide Accession Num NM_003268
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBAZ2
Species Reactivity Human