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TMEM27 ELISA Kit (Human) (GWB-KBBQW7)

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GWB-KBBQW7

96 Wells
$709.00
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Additional Information

Name TMEM27 ELISA Kit (Human) (GWB-KBBQW7)
Description of Target This gene encodes a type 1 transmembrane protein that is important for trafficking amino acid transporters to the apical brush border of proximal tubules. The encoded protein binds to amino acid transporters and regulates their expression on the plasma me
Kit Range 0.156-10 ng/mL
Sensitivity < 0.057 ng/mL
Sample Type Serum, plasma, tissue homogenates, cell lysates, cell culture supernatant.
Kit Detection Absorbance, OD 450 nm
Gene Symbol TMEM27
Gene id 57393
Alias Symbols Collectrin, NX17, NX-17, Transmembrane protein 27, UNQ679/PRO1312
Swissprot ID Q9HBJ8
Protein Name Collectrin
Kit Principle Genway Biotech TMEM27 ELISA Kit (Human) (GWB-KBBQW7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TMEM27 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TMEM27 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TMEM27 captured in the well.
Kit Component
ComponentAmount
Anti-TMEM27 Microplate96 Wells (12 x 8 Well Strips)
TMEM27 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X TMEM27 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_065716.1
Nucleotide Accession Num NM_020665.5
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBQW7
Species Reactivity Human