TMEM2L ELISA Kit (Human) (GWB-KBBYY7)

GWB-KBBYY7

96 Wells
$645.00
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Additional Information

Name TMEM2L ELISA Kit (Human) (GWB-KBBYY7)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.052 ng/mL
Sample Type Serum, plasma, tissue homogenates, cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol TMEM2L
Gene id 57214
Alias Symbols CCSP1, IR2155535, Protein KIAA1199, TMEM2L
Swissprot ID Q8WUJ3
Protein Name Cell migration-inducing and hyaluronan-binding protein
Description of Target Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin-coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that m
Kit Principle Genway Biotech TMEM2L ELISA Kit (Human) (GWB-KBBYY7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TMEM2L has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TMEM2L is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TMEM2L captured in the well.
Kit Component
ComponentAmount
Anti-TMEM2L Microplate96 Wells (12 x 8 Well Strips)
TMEM2L Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X TMEM2L Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001280227.1
Nucleotide Accession Num NM_001293298.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBYY7