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96 Wells
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Additional Information

Name TNFSF9 ELISA Kit (Human) (GWB-KBBMN1)
Description of Target Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.
Kit Range 7.8 - 500 pg/mL
Sensitivity < 2.7 pg/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol TNFSF9
Gene id 8744
Alias Symbols 4-1BBL, 4-1BB-L, 4-1BB ligand, CD137L, Tumor necrosis factor ligand superfamily member 9
Swissprot ID P41273
Protein Name Tumor necrosis factor ligand superfamily member 9
Kit Principle Genway Biotech TNFSF9 ELISA Kit (Human) (GWB-KBBMN1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNFSF9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TNFSF9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TNFSF9 captured in the well.
Kit Component
Anti-TNFSF9 Microplate96 Wells (12 x 8 Well Strips)
TNFSF9 Lyophilized Standard2 x 500 pg
100X Biotinylated TNFSF9 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_003802
Nucleotide Accession Num NM_003811
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)95-102%84-103%80-94%98-105%
EDTA Plasma (n=5)86-94%80-96%87-103%93-101%
Heparin Plasma (n=5)95-102%97-105%87-94%95-102%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)85-9590
EDTA Plasma (n=5)98-105102
Heparin Plasma (n=5)87-9693
Datasheets/Manuals Printable datasheet for GWB-KBBMN1
Species Reactivity Human