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TP63 ELISA Kit (Mouse) (GWB-KBBBG4)

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GWB-KBBBG4

96 Wells
$719.00
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Additional Information

Name TP63 ELISA Kit (Mouse) (GWB-KBBBG4)
Description of Target This gene encodes tumor protein p63, a member of the p53 family of transcription factors involved in cellular responses to stress and development. the family members include tumor proteins p53, p63, and p73, which have high sequence similarity to one anot
Kit Range 0.156-10 ng/mL
Sensitivity <0.054ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol TP63
Gene id 22061
Alias Symbols AI462811, deltaNp63, Ket, KET protein, MGC115972, p51/p63, P51/P63, p63, P63, p73L, P73l, TAp63, Tp63, TP63, Tp73l, Transformation-related protein 63, Trp53rp1, Tumor protein 63, Tumor protein p73-like
Swissprot ID O88898
Protein Name tumor protein 63
Kit Principle Genway Biotech TP63 ELISA Kit (Mouse) (GWB-KBBBG4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TP63 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TP63 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TP63 captured in the well.
Kit Component
ComponentAmount
Anti-TP63 Microplate96 Wells (12 x 8 Well Strips)
TP63 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X TP63 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001120731.1
Nucleotide Accession Num NM_001127259.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBBG4
Species Reactivity Mouse