Triiodothyronine (T3) ELISA Quantitation Kit Manual

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Species: Human

Sample Type: Serum

For the quantitative determination of Triiodothyronine (T3) in serum. Blood, Thyroid Diseases, Endocrine Glands, Liver, Central Nervous System Please click here for MSDS PDF Datasheet

Product Name: Triiodothyronine (T3)

Sample Type: Serum

# of Samples: 1X96 Assays

Intended Use: The GenWay Biotech Total T3 EIA is intended for the quantitative determination of triiodothyronine (T3) concentration in human serum.

Introduction: The thyroid hormones, thyroxine (T4) and 3, 5, 3\' triiodothyronine (T3) circulate in the bloodstream mostly bound to the plasma protein, thyroxine binding globulin (TBG).1 The concentration of T3 is much less than that of T4, but its metabolic potency is much greater.^2,3 These as well as other hormones are produced by the thyroid gland, situated at the level of the thyroid cartilage on either side of the larynx. The thyroid gland and associated hormones are a major component of the endocrine system. They exert powerful and essential regulatory influences on growth, differentiation, cellular metabolism, and general hormonal balance of the body, as well as on the maintenance of metabolic activity and the development of the skeletal and organ systems.
T3 measurement has uncovered a variant of hyperthyroidism in thyrotoxic samples with elevated T3 values and normal T4 values.^5 Likewise, an increase in T3 without an increase in T4 is frequently a forerunner of recurrent thyrotoxicosis in previously treated samples. In addition to hyperthyroidism, T3 levels are elevated in women who are pregnant, and in women receiving oral contraceptives or estrogen treatment.

Principles of the assay: In the GenWay Biotech T3 EIA, a second antibody (goat anti-mouse IgG) is coated on a microtiter wells. A measured amount of serum, a certain amount of mouse monoclonal Anti-T3 antibody, and a constant amount of T3 conjugated with horseradish peroxidase are added to the microtiter wells. During incubation, the mouse anti-T3 antibody is bound to the second antibody on the wells. T3 and the enzyme conjugated-T3 compete for the limited binding sites on the anti-T3 antibody. After a 60 minute incubation at room temperature, the wells are washed 5 times by water to remove unbound T3 conjugate. A solution of TMB is then added and incubated for 20 minutes at room temperature, resulting in the development of a blue color. The color development is stopped with the addition of 1N HCl, and the absorbance is measured spectrophotometrically at 450 nm. The intensity of the color formed is proportional to the amount of enzyme present, and is inversely related to the amount of unlabeled T3 standards assayed in the same way. The concentration of T3 in the unknown sample is then calculated.^7-11

Storage and Stability: 1. Store the unopened kit at 2-8C upon receipt and when not in use, until the expiration shown on the kit label. Refer to the package label for the expiration date.
2. Keep microtiter plate in a sealed bag with desiccant to minimize exposure to damp air.

Limitation of the Test: 1. Important Note: Individuals receiving thyroid replacement therapy, such as triiodothyroacetic acid or triiodothyropionic acid, may give falsely high T3 values in this test. Numerous other conditions unrelated to thyroid disease may cause abnormal T3 values (see Expected Results).
2. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the package insert instructions and with adherence to good laboratory practice.
3. Serum samples with T3 concentrations greater than 10 ng/mL should be diluted with the Zero Standard to fit into the assay range, and re-assayed. The obtained value should then be multiplied by the dilution factor to obtain the true serum value.
4. Icteric samples with bilirubin values as high as 5 mg/dl do not affect the assay.
5. Added hemoglobin levels of up to 100 mg/dl showed no effect on the T3 value.
6. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings.

Product Name: Triiodothyronine (T3)

Additional Information

Name Triiodothyronine (T3) ELISA Quantitation Kit Manual
Related Product Names Triiodothyronine (T3) ELISA Kit Triiodothyronine (T3)T3
Intended Use Research Use Only