The GenWay, Inc. Triiodothyronine (T3) ELISA Kit is intended for the detection of total T3 in human serum or plasma.
SUMMARY AND EXPLANATION
T3 is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T3 is decreased in hypothyroid patients and is increased in hyperthyroid patients. The level of T3 is normal in Euthyroidindividuals.
PRINCIPLE OF THE TEST
The GenWay T3 is a solid phase competitive ELISA. The samples and the diluted T3 enzyme conjugate are added to the wells coated with anti-T3 polyclonal antibody. T3 in the patient’s serum competes with a T3 enzyme (HRP) conjugate for binding sites. Unbound T3 and T3 enzyme conjugate is washed off by wash buffer during a wash step. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T3 in the samples. A standard curve generated relating color intensity to the concentration of the T3.