Tuba4a ELISA Kit (Mouse) (GWB-KBBXL7)

GWB-KBBXL7

96 Wells
$600.00
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Additional Information

Name Tuba4a ELISA Kit (Mouse) (GWB-KBBXL7)
Availability 0
Description of Target Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.124 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol TUBA4A
Gene id 22145
Alias Symbols Alpha-tubulin 4, Alpha-tubulin isotype M-alpha-4, M[a]4, Tuba4, Tubulin alpha-4A chain, Tubulin alpha-4 chain
Swissprot ID P68368
Protein Name Tubulin alpha-4A chain
Kit Principle Genway Biotech Tuba4a ELISA Kit (Mouse) (GWB-KBBXL7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tuba4a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tuba4a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tuba4a captured in the well.
Kit Component
ComponentAmount
Anti-Tuba4a Microplate96 Wells (12 x 8 Well Strips)
Tuba4a Lyophilized Standard2 x 20 ng
100X Biotinylated Tuba4a Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_033473
Nucleotide Accession Num NM_009447
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBXL7
Species Reactivity Mouse