UBA52 ELISA Kit (Human) (GWB-KBBJS1)

This exact product is now provided by Aviva Systems Biology.
Please visit OKCD01827 to review further and for ordering information.


96 Wells
Add to Cart

Additional Information

Name UBA52 ELISA Kit (Human) (GWB-KBBJS1)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.116 ng/mL
Sample Type tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol UBA52
Gene id 7311
Alias Symbols CEP52, HUBCEP52, L40, MGC126879, MGC126881, MGC57125, RPL40
Swissprot ID P62987
Protein Name Ubiquitin-60S ribosomal protein L40
Description of Target Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of th
Kit Principle Genway Biotech UBA52 ELISA Kit (Human) (GWB-KBBJS1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for UBA52 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for UBA52 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample UBA52 captured in the well.
Kit Component
Anti-UBA52 Microplate96 Wells (12 x 8 Well Strips)
UBA52 Lyophilized Standard2 x 20 ng
100X Biotinylated UBA52 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001029102
Nucleotide Accession Num NM_001033930
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBJS1
Species Reactivity Human