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96 Wells
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Additional Information

Name UQCRC2 ELISA Kit (Mouse) (GWB-KBBWD5)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.063 ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol UQCRC2
Gene id 67003
Alias Symbols 1500004O06Rik, Complex III subunit 2, Core protein II, Cytochrome b-c1 complex subunit 2, mitochondrial, Ubiquinol-cytochrome-c reductase complex core protein 2
Swissprot ID Q9DB77
Protein Name cytochrome b-c1 complex subunit 2, mitochondrial
Description of Target This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex (
Kit Principle Genway Biotech UQCRC2 ELISA Kit (Mouse) (GWB-KBBWD5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for UQCRC2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for UQCRC2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample UQCRC2 captured in the well.
Kit Component
Anti-UQCRC2 Microplate96 Wells (12 x 8 Well Strips)
UQCRC2 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X UQCRC2 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_080175.1
Nucleotide Accession Num NM_025899.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBWD5
Species Reactivity Mouse