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USP6NL ELISA Kit (Human) (GWB-KBBJZ3)

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GWB-KBBJZ3

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Additional Information

Name USP6NL ELISA Kit (Human) (GWB-KBBJZ3)
Description of Target Acts as a GTPase-activating protein for RAB5A and RAB43. Involved in receptor trafficking. In complex with EPS8 inhibits internalization of EGFR. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.058 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol USP6NL
Gene id 9712
Alias Symbols KIAA0019, Related to the N-terminus of tre, RNTRE, RN-tre, TRE2NL, USP6 N-terminal-like protein
Swissprot ID Q92738
Protein Name USP6 N-terminal-like protein
Kit Principle Genway Biotech USP6NL ELISA Kit (Human) (GWB-KBBJZ3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for USP6NL has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for USP6NL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample USP6NL captured in the well.
Kit Component
ComponentAmount
Anti-UBIAD1 Microplate96 Wells (12 x 8 Well Strips)
UBIAD1 Lyophilized Standard2 x 10 ng
100X Biotinylated UBIAD1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001073960
Nucleotide Accession Num NM_001080491
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBJZ3
Species Reactivity Human