Zika Virus ELISA Kit - IgM

GWB-ZKAEK1

96 Wells
$599.00
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Description

The Zika Virus IgM μ-capture ELISA is intended for the qualitative determination of IgM class antibodies against Zika virus in human serum or plasma (citrate, heparin).

Introduction:

Zika Virus (ZIKV) is a single-stranded RNA virus of the Flaviviridae family (genus Flavivirus). It was first isolated in 1947 from a sentinel rhesus monkey during a yellow fever study in the Zika forest of Uganda.
Since its discovery, ZIKV circulation has been detected in Africa and Asia where it has caused sporadic human infections. In 2007 its emergence on Yap Island, Micronesia was reported, marking transmission of Zika virus outside Africa and Asia. Since 2013, ZIKV has been reported from French Polynesia, New Caledonia, Cook Islands, Easter Island (Chile), Samoa and Vanuatu, and in early 2015 it spread initially to Brazil and subsequently to additional countries of the Americas.
ZIKV is transmitted primarily through the bite of an infected Aedes species mosquito (A. aegypti and A. albopictus). However, there have been reports of less common transmission modes, such as blood transfusion, perinatal, and sexual contact.
The incubation period of Zika virus disease is not known precisely, but is likely to be a few days.
It is estimated that only one in five people infected with ZIKV develop signs or symptoms. Clinical manifestations of ZIKV infection are described as very similar to those of Dengue virus (DENV) and Chikungunya virus (CHIKV) infections, but usually milder.
The most common clinical signs and symptoms are maculopapular rash, low grade fever, arthralgia, myalgia, headache and conjunctivitis. Less frequently reported are oedema, sore throat, cough, vomiting, and haematospermia.
Human infections with ZIKV are usually mild and self-limiting and the symptoms usually resolve spontaneously after 3–7 days; arthralgia may persist for up to 1 month. In rare cases, after a Zika virus infection a Guillain-Barré syndrome (GBS), a disorder of the peripheral nerves, can probably occur. A correlation between a Zika virus infection in pregnancy and congenital brain malformations is now considered likely.

Assay Principle:

The qualitative immunoenzymatic determination of specific IgM-class antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) μ-capture technique.
Microplates are coated with anti-human IgM-class antibodies to bind the corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled Zika virus antigen is added. This antigen-conjugate binds to the captured specific IgM antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product.
The intensity of this product is proportional to the amount of specific IgM antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader.

Additional Information

Name Zika Virus ELISA Kit - IgM
Sample Type Serum
Datasheets / Downloads Click here to download product manual.
Concentration Lot Specific
Applications ELISA
Format 12 break-apart 8-well snap-off strips coated with anti-human IgM-class antibodies.
Reactivity Human (serum)
Storage Store the kit at 2-8 °C. The opened reagents are stable up to the expiry date stated on the label when stored at 2-8 °C.
Intended Use Research Use Only