| Name |
Zp2 ELISA Kit (Rat) (GWB-KBBUU3) |
| Description of Target |
The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sper |
| Kit Range |
0.312 - 20 ng/mL |
| Sensitivity |
< 0.125 ng/mL |
| Sample Type |
tissue homogenates, cell lysates and other biological fluids. |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
ZP2 |
| Gene id |
81828 |
| Alias Symbols |
Zona pellucida glycoprotein 2, Zona pellucida protein A, Zona pellucida sperm-binding protein 2, Zp-2, Zpa |
| Swissprot ID |
O54767 |
| Protein Name |
zona pellucida sperm-binding protein 2 |
| Kit Principle |
Genway Biotech Zp2 ELISA Kit (Rat) (GWB-KBBUU3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Zp2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Zp2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Zp2 captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-NPM1 Microplate | 96 Wells (12 x 8 Well Strips) | | NPM1 Lyophilized Standard | 2 x 20 ng | | 100X Biotinylated NPM1 Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_112412 |
| Nucleotide Accession Num |
NM_031150 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBUU3 |
| Species Reactivity |
Rat |
