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Zp2 ELISA Kit (Rat) (GWB-KBBUU3)

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96 Wells
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Additional Information

Name Zp2 ELISA Kit (Rat) (GWB-KBBUU3)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.125 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ZP2
Gene id 81828
Alias Symbols Zona pellucida glycoprotein 2, Zona pellucida protein A, Zona pellucida sperm-binding protein 2, Zp-2, Zpa
Swissprot ID O54767
Protein Name zona pellucida sperm-binding protein 2
Description of Target The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sper
Kit Principle Genway Biotech Zp2 ELISA Kit (Rat) (GWB-KBBUU3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Zp2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Zp2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Zp2 captured in the well.
Kit Component
Anti-NPM1 Microplate96 Wells (12 x 8 Well Strips)
NPM1 Lyophilized Standard2 x 20 ng
100X Biotinylated NPM1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_112412
Nucleotide Accession Num NM_031150
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBUU3
Species Reactivity Rat