ZYX ELISA Kit (Human) (GWB-KBBQV7)

GWB-KBBQV7

96 Wells
$580.00
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Additional Information

Name ZYX ELISA Kit (Human) (GWB-KBBQV7)
Availability In Stock
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.061 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ZYX
Gene id 7791
Alias Symbols ESP-2, HED-2, Zyxin, Zyxin-2
Swissprot ID Q15942
Protein Name zyxin
Description of Target Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates
Kit Principle Genway Biotech ZYX ELISA Kit (Human) (GWB-KBBQV7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ZYX has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ZYX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ZYX captured in the well.
Kit Component
ComponentAmount
Anti-FUS Microplate96 Wells (12 x 8 Well Strips)
FUS Lyophilized Standard2 x 10 ng
100X Biotinylated FUS Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001010972
Nucleotide Accession Num NM_001010972
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBQV7
Species Reactivity Human